Update - 4.30pm:
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One southbound lane of the Hume Motorway has reopened at Yerrinbool after a truck crash.
One southbound lane of the motorway remains closed at Sierra Street as work continues to clean-up the crash site.
Southbound traffic remains very heavy and is queued for around seven kilometres so motorists are still advised to allow plenty of extra time to travel through the area.
Update - 3.20pm:
Motorists are able to pass the site of a truck crash in the southbound lanes of the Hume Motorway at Yerrinbool.
Two southbound lanes of the motorway remain closed at Sierra Street, with motorists using the shoulder of the road to get past the crash.
Southbound traffic remains very heavy and motorists are advised to allow plenty of extra travel time as there are significant delays.
Emergency services and traffic crews are still on site, working to clear the site.
A Transport Management Centre spokesman said traffic was "moving slowly" and backed up for about six kilometres.
Earlier - 1.50pm:
A truck and car crash has closed the Hume Motorway in a southbound direction.
The accident, which happened just before 2pm on Tuesday, happened near Sierra Street, Yerrinbool.
Transport for NSW crews and emergency services are in attendance.
Motorists are advised to expect delays and exercise caution.
Light vehicles can exit the motorway at Avon Dam Rd and use Remembrance Driveway/Old Hume Hwy as an alternative.
Heavy vehicles are being parked.