The crash site has been cleared by tow truck drivers and emergency services.
The cause of the crash is unknown, according to police.
A single-vehicle crash occurred northbound in Aylmerton this morning.
The crash, which happened on the Hume Motorway at Old Hume Highway, happened just after 10.30am on Monday.
A Transport Management Centre spokesman said the vehicle has rolled however no one was trapped and there were no injuries.
The spokesman said there were no significant delays in the northbound lanes, however there appeared to be some southbound delays.
He said this could be due to roadworks.
Emergency services are in attendance. Motorists are advised to reduce their speed and exercise caution.