Goulburn Police said that no one had been injured and the road was now open. Officers are directing traffic.
A multi-car crash has closed one northbound lane on the Hume Highway at Sallys Corner Road.
The crash, which happened at about 4.45pm on Monday, reportedly occurred between five cars.
Police and Rural Fire Service crews are at the scene, five kilometres south of the McDonald's restaurant on the northbound side of the highway.
Motorists are advised to allow additional travel time and exercise caution as there is fluid on the road.