Two people are injured and a massive clean-up operation is underway in Berrima after a truck rolled onto its side on the Hume Highway on Tuesday night.
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It is understood the truck spilled diesel over the highway after an accident involving another truck around 6pm.
"There was a large fuel spill, about 500 litres, which Fire and Rescue were containing and cleaning up using absorbent material," a FRNSW spokesman said.
"They were monitoring run off-in conjunction with EPA."
The RFS assisted with fire protection.
An Ambulance NSW spokeswoman said one driver, a 48-year-old male, was conscious, breathing and had a possible injured leg when he was assessed by paramedics.
Another person who was trapped inside one of the trucks was freed by SES, before being transported by ambulance to hospital.
Police are in attendance.
Live Traffic advises southbound lanes are closed for a short time to salvage the truck.
The northbound direction is affected by moderate traffic conditions.
Live Traffic advises motorists to exercise caution and allow extra travel time.
Visit Live Traffic for the latest updates.